In vitro prototyping and rapid optimization of biosynthetic enzymes for cellular design
Ashty S. Karim, Quentin M. Dudley, Alex Juminaga, Yongbo Yuan, Samantha A. Crowe, Jacob T. Heggestad, Shivani Garg, Tanus Abdalla, William S. Grubbe, Blake J. Rasor, David N. Coar, Maria Torculas, Michael P. Krein, Fungmin Eric Liew, Amy Quattlebaum, , Michael C. Jewett
The paper introduces iPROBE, a platform for in vitro prototyping and rapid optimization of biosynthetic enzymes, demonstrating improved production of 3-hydroxybutyrate and n-butanol in microbial cell factories.
Nature Chemical Biology · Jun 15, 2020Peer-reviewedSynthetic Biology