Cell-free prototyping enables implementation of optimized reverse beta-oxidation pathways in heterotrophic and autotrophic bacteria
Bastian Vögeli, Luca Schulz, Shivani Garg…·Michael C. Jewett
The paper optimizes and implements reverse beta-oxidation pathways in bacteria for carbon-negative synthesis of C4-C6 acids and alcohols, achieving high product titers in engineered strains.