Author

N. D. Holliday

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 2 Cell Biology· 1 Pharmacology & Toxicology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Research Focus:cell type annotation· 2 Organism:human· 2 Research Focus:protein folding & dynamics· 2 Assay:proteomics· 2 Research Focus:host-pathogen & immune response· 1 Organism:mouse· 1 Assay:protein structure· 1

Frequent Co-Authors

S. J. Briddon· 2 shared A. Di Maio· 1 shared A. Kaur· 1 shared A. L. Matthews· 1 shared A. Malinova· 1 shared B. Cragoe· 1 shared B. Kellam· 1 shared B. L. Hoare· 1 shared

Activity by Year

2019-2023 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 17 mutual co-author links

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2 shared papersBiochemistry

S. J. Briddon

Mutual co-authors in cluster

Author profile →

4 papers

Recent·Most cited

A time-resolved Forster resonance energy transfer assay to investigate inhibitor binding to ABCG2

J. I. Mitchell-White, D. A. Briggs, S. J. Mistry, H. A. Mbiwan, B. Kellam, N. D. Holliday, S. J. Briddon, I. D. Kerr

The paper introduces a TR-FRET assay to measure inhibitor binding to ABCG2 and confirms NBD dimerization correlates with a shift in inhibitor affinity.

bioRxiv · Oct 27, 2023 · PreprintBiochemistry

Development of fluorescent peptide G Protein Coupled Receptor activation biosensors for NanoBRET characterisation of intracellular allosteric modulators

J. P. Farmer, S. N. Mistry, C. A. Laughton, N. D. Holliday

This paper develops fluorescent peptide biosensors for NanoBRET-based assessment of GPCR intracellular allosteric modulator (IAM) interactions, demonstrating their utility in affinity screening and receptor activation studies.

bioRxiv · May 30, 2022 · PreprintPharmacology & Toxicology

ThermoBRET: a ligand-engagement nanoscale thermostability assay applied to GPCRs

B. L. Hoare, D. N. Tippett, A. Kaur, S. A. Cullum, T. Miljus, E. J. Koers, C. R. Harwood, N. Dijon, N. D. Holliday, D. A. Sykes, D. B. Veprintsev

ThermoBRET quantifies GPCR thermostability using BRET without labelled ligands, revealing ligand effects on protein unfolding in non-purified samples.

bioRxiv · Aug 5, 2020Biochemistry

Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex

C. Z. Koo, N. Harrison, P. J. Noy, J. Szyroka, A. L. Matthews, H.-E. Hsia, S. A. Mueller, J. Tushaus, J. Goulding, K. Willis, C. Apicella, B. Cragoe, E. Davis, M. Keles, A. Malinova, , M. G. Tomlinson

The paper identifies Tspan15 as a critical partner of ADAM10, showing their interaction and functional synergy in proteolytic activity.

bioRxiv · Oct 11, 2019Cell Biology