Author

D. B. Veprintsev

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 3 Pharmacology & Toxicology· 3

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Research Focus:protein folding & dynamics· 3 Assay:protein structure· 3 Research Focus:cell signaling & trafficking· 2 Organism:human· 2 Research Focus:cell type annotation· 1 Research Focus:drug response / drug discovery· 1 Organism:E. coli· 1

Frequent Co-Authors

C. R. Harwood· 3 shared D. A. Sykes· 3 shared F. M. Heydenreich· 2 shared S. J. Briddon· 2 shared T. Miljus· 2 shared A. C. Rufer· 1 shared A. Kaur· 1 shared A. Kimbara· 1 shared

Activity by Year

2017-2021 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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3 shared papersBiochemistry

C. R. Harwood

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Author profile →

6 papers

Recent·Most cited

Exploring the kinetic selectivity of drugs targeting the β1-adrenoceptor

D. A. Sykes, M. Jimenez Roses, J. Reilly, R. A. Fairhurst, S. J. Charlton, D. B. Veprintsev

This study investigates the kinetic selectivity of drugs targeting the β1-adrenoceptor, finding that hydrophobic interactions dominate ligand binding kinetics over combined polar and hydrophobic interactions.

bioRxiv · Aug 31, 2021Pharmacology & Toxicology

Diverse chemotypes drive biased signaling by cannabinoid receptors

T. Miljus, F. M. Heydenreich, T. Gazzi, A. Kimbara, M. Rogers-Evans, M. Nettekoven, E. Zirwes, A. Osterwald, A. C. Rufer, C. Ullmer, W. Guba, C. Le Gouill, J. Fingerle, M. Nazare, U. Grether, , D. B. Veprintsev

This study identifies chemotype-specific biased signaling in cannabinoid receptors by analyzing ligand-induced signaling cascades.

bioRxiv · Nov 9, 2020 · PreprintPharmacology & Toxicology

ThermoBRET: a ligand-engagement nanoscale thermostability assay applied to GPCRs

B. L. Hoare, D. N. Tippett, A. Kaur, S. A. Cullum, T. Miljus, E. J. Koers, C. R. Harwood, N. Dijon, N. D. Holliday, D. A. Sykes, D. B. Veprintsev

ThermoBRET quantifies GPCR thermostability using BRET without labelled ligands, revealing ligand effects on protein unfolding in non-purified samples.

bioRxiv · Aug 5, 2020Biochemistry

Functional solubilisation of the β2-adrenoceptor (β2AR) using Diisobutylene maleic acid (DIBMA).

C. R. Harwood, D. A. Sykes, B. Hoare, F. M. Heydenreich, R. Uddin, D. R. Poyner, S. J. Briddon, D. B. Veprintsev

This paper demonstrates the functional solubilisation of the β2-adrenoceptor using DIBMA, showing improved thermal stability compared to detergent-based methods.

bioRxiv · Jun 29, 2020Biochemistry

Ligand-directed covalent labelling of a GPCR with a fluorescent tag

L. A. Stoddart, N. D. Kindon, O. Otun, C. R. Harwood, F. Patera, D. B. Veprintsev, J. Woolard, S. J. Briddon, H. A. Franks, S. J. Hill, B. Kellam

The paper describes a compound that covalently labels a GPCR with a fluorescent tag without blocking its binding site, enabling study of native GPCR function and localization.

bioRxiv · Apr 21, 2020Pharmacology & Toxicology

Production and purification of human Hsp90β in Escherichia coli

M. Radli, D. B. Veprintsev, S. G. D. Rudiger

This paper optimizes the production and purification of recombinant human Hsp90β in E. coli to yield a stable, pure, and well-folded protein.

bioRxiv · Mar 30, 2017Biochemistry