Author

S. J. Briddon

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Pharmacology & Toxicology· 5 Biochemistry· 2 Cell Biology· 2

Signature Assays, Organisms & Methods

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Approach:experimental assay· 7 Organism:human· 6 Assay:proteomics· 5 Research Focus:cell signaling & trafficking· 4 Research Focus:cell type annotation· 3 Research Focus:protein folding & dynamics· 3 Approach:biophysical modeling· 1 Research Focus:clustering· 1

Frequent Co-Authors

B. Kellam· 3 shared C. R. Harwood· 3 shared D. A. Sykes· 2 shared D. B. Veprintsev· 2 shared J. Goulding· 2 shared J. Woolard· 2 shared N. D. Holliday· 2 shared A. Batista-Gondin· 1 shared

Activity by Year

2019-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 15 mutual co-author links

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3 shared papersPharmacology & Toxicology

B. Kellam

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Author profile →

9 papers

Recent·Most cited

Agonist efficacy at the B2AR is driven by agonist-induced differences in receptor affinity for the Gs protein, not ligand binding kinetics.

C. R. Harwood, D. A. Sykes, T. Redfern-Nichols, G. Ladds, S. J. Briddon, D. Veprintsev

This study shows that agonist efficacy at the {beta}2AR is determined by differences in receptor affinity for Gs protein, not ligand binding kinetics.

bioRxiv · Jan 5, 2024 · PreprintPharmacology & Toxicology

Key phosphorylation sites for robust β-arrestin2 binding at the mu-opioid receptor revisited

O. Underwood, S. Frizwanker, J. Glenn, A. Batista-Gondin, J. Drube, C. Hoffmann, S. J. Briddon, S. S. Schulz, M. Canals

The paper investigates the role of GRK subfamilies in phosphorylation of the MOR C-tail and their impact on β-arrestin2 recruitment, showing GRK2/3 and GRK5/6 have distinct contributions.

bioRxiv · Dec 5, 2023 · PreprintPharmacology & Toxicology

A time-resolved Forster resonance energy transfer assay to investigate inhibitor binding to ABCG2

J. I. Mitchell-White, D. A. Briggs, S. J. Mistry, H. A. Mbiwan, B. Kellam, N. D. Holliday, S. J. Briddon, I. D. Kerr

The paper introduces a TR-FRET assay to measure inhibitor binding to ABCG2 and confirms NBD dimerization correlates with a shift in inhibitor affinity.

bioRxiv · Oct 27, 2023 · PreprintBiochemistry

Simple methods for quantifying super-resolved cortical actin

E. Garlick, E. L. Faulkner, S. J. Briddon, S. G. Thomas

This paper develops and validates methods to quantify super-resolved cortical actin structures using SRRF and ExM, showing increased corral area with cytochalasin D treatment.

bioRxiv · May 26, 2021Cell Biology

Acylation of the incretin peptide exendin-4 directly impacts GLP-1 receptor signalling and trafficking

M. Lucey, T. Ashik, A. Marzook, Y. Wang, J. Goulding, A. Oishi, J. Broichhagen, D. Hodson, J. Minnion, Y. Elani, R. Jockers, S. J. Briddon, S. R. Bloom, A. Tomas, B. Jones

This study compares exendin-4 and its acylated variant exendin-4-C16, showing acylation affects GLP-1R signaling bias, trafficking, and pharmacokinetics.

bioRxiv · Apr 1, 2021Pharmacology & Toxicology

Functional solubilisation of the β2-adrenoceptor (β2AR) using Diisobutylene maleic acid (DIBMA).

C. R. Harwood, D. A. Sykes, B. Hoare, F. M. Heydenreich, R. Uddin, D. R. Poyner, S. J. Briddon, D. B. Veprintsev

This paper demonstrates the functional solubilisation of the β2-adrenoceptor using DIBMA, showing improved thermal stability compared to detergent-based methods.

bioRxiv · Jun 29, 2020Biochemistry

Ligand-directed covalent labelling of a GPCR with a fluorescent tag

L. A. Stoddart, N. D. Kindon, O. Otun, C. R. Harwood, F. Patera, D. B. Veprintsev, J. Woolard, S. J. Briddon, H. A. Franks, S. J. Hill, B. Kellam

The paper describes a compound that covalently labels a GPCR with a fluorescent tag without blocking its binding site, enabling study of native GPCR function and localization.

bioRxiv · Apr 21, 2020Pharmacology & Toxicology

Monitoring ligand-induced changes in receptor conformation with NanoBiT conjugated nanobodies

M. Soave, R. Heukers, B. Kellam, J. Woolard, M. J. Smit, S. J. Briddon, S. Hill

The paper uses nanobodies conjugated with NanoBiT to monitor ligand-induced conformational changes in the CXCR4 receptor.

bioRxiv · Apr 21, 2020 · PreprintPharmacology & Toxicology

Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex

C. Z. Koo, N. Harrison, P. J. Noy, J. Szyroka, A. L. Matthews, H.-E. Hsia, S. A. Mueller, J. Tushaus, J. Goulding, K. Willis, C. Apicella, B. Cragoe, E. Davis, M. Keles, A. Malinova, , M. G. Tomlinson

The paper identifies Tspan15 as a critical partner of ADAM10, showing their interaction and functional synergy in proteolytic activity.

bioRxiv · Oct 11, 2019Cell Biology