N. Merindol

T7 RNA polymerase-independent expression of reporter genes from a T7 promoter-driven SARS-CoV-2 replicon-encoding DNA in human cells

This study shows that a SARS-CoV-2 replicon DNA can express reporter genes in human cells without requiring T7 RNA polymerase or RNA replication.

Characterization of norbelladine synthase and noroxomaritidine/norcraugsodine reductase reveals a novel catalytic route for the biosynthesis of Amaryllidaceae alkaloids including the Alzheimer's drug galanthamine

The paper characterizes two enzymes involved in the biosynthesis of Amaryllidaceae alkaloids, showing they work together to produce norbelladine, a precursor to the Alzheimer's drug galanthamine.

Editing of the TRIM5 gene decreases the permissiveness of human T lymphocytic cells to HIV-1

This study edits the TRIM5 gene in human T cells to restrict HIV-1 infection, showing biallelic modification is needed for significant inhibition.

Editing of the Human TRIM5 Gene to Introduce Mutations with the Potential to Inhibit HIV-1

This study edits the human TRIM5 gene to introduce HIV-1 inhibitory mutations but finds limited restriction activity in HEK293T cells.

Gene knockout shows that PML (TRIM19) does not restrict the early stages of HIV-1 infection in human cell lines

This paper uses CRISPR-Cas9 to knockout PML in human cell lines and shows it does not restrict early HIV-1 infection or IFN-I-mediated inhibition.