P. Dupree

Evolution of glucuronoxylan side chain variability in vascular plants and the counter-adaptation of pathogenic cell-wall-degrading hydrolases

The paper identifies the genetic basis of xylan side chain variability in plants and shows how pathogens adapt to degrade these structures, revealing evolutionary adaptations in both biosynthesis and degradation.

Fluorogenic, sub-single-turnover monitoring of enzymatic reactions involving NAD(P)H provides a generalised platform for directed ultrahigh throughput evolution of biocatalysts in microdroplets

The paper develops a fluorogenic assay for NAD(P)H detection to enable high-throughput screening of biocatalysts in microdroplets, demonstrating improved sensitivity and applicability for glycosyl hydrolases and IREDs.

Altering the substitution and crosslinking of glucuronoarabinoxylans affects cell wall porosity and assembly in Brachypodium distachyon.

This study shows that altering xylan biosynthesis in Brachypodium distachyon affects cell wall porosity and structure, impacting digestibility and biomass properties.

Differing structures of galactoglucomannan in eudicots and non-eudicot angiosperms

This paper investigates the structural differences of galactoglucomannan in eudicots and non-eudicot angiosperms, finding that {beta}-GGM is likely eudicot-specific.

The biosynthesis, degradation, and function of cell wall β-xylosylated xyloglucan mirrors that of arabinoxyloglucan

The paper identifies enzymes involved in the biosynthesis and degradation of β-xylosylated xyloglucan and shows their potential role in gut bacterial nutrition.

Eudicot primary cell wall glucomannan is related in synthesis, structure and function to xyloglucan

The paper shows that β-galactoglucomannan (β-GGM) in eudicot primary cell walls is structurally and functionally similar to xyloglucan (XyG).

Functional metagenomic screening in microfluidic droplets identifies a β-glucuronidase in an unprecedented sequence neighbourhood

This study identifies a novel β-glucuronidase from a metagenomic library using microfluidic droplet screening, revealing a new GH3 family member with unique functional and structural properties.

Discovery of putative Golgi S-Adenosyl methionine transporters reveals the importance of plant cell wall polysaccharide methylation

This study identifies putative Golgi SAM transporters in Arabidopsis and shows their role in cell wall polysaccharide methylation and plant growth.

Two softwood GUX clades are responsible for distinct glucuronic acid patterns on xylan.

The paper identifies two distinct GUX clades in softwood that produce different glucuronic acid patterns on xylan, affecting biomass recalcitrance.

Structural imaging of native cryo-preserved secondary cell walls reveals presence of macrofibrils composed of cellulose, lignin and xylan.

This study uses cryo-SEM to image native secondary cell walls, revealing macrofibrils composed of cellulose, xylan, and lignin in both angiosperm and gymnosperm species.

Expression of a glucomannan mannosyltransferase gene (GMMT) from Aloe vera is induced by water deficit and abscisic acid.

This study shows that the GMMT gene in Aloe vera is upregulated by water stress and exogenous ABA, suggesting ABA regulation of acemannan synthesis.

A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of A Secondary Cell Wall Integrity System in Arabidopsis

This study analyzes the transcriptome of xylan mutants in Arabidopsis to test for a secondary cell wall integrity system, finding minimal transcriptomic changes.