P. Avasthi

Determinants of cytoplasmic microtubule depolymerization during ciliogenesis in Chlamydomonas reinhardtii

This study investigates the role of cytoplasmic microtubule dynamics during ciliogenesis in Chlamydomonas reinhardtii using chemical and mechanical perturbations, finding that CytoMT depolymerization is a consequence, not a requirement, for ciliogenesis.

Lithium-induced ciliary lengthening sparks Arp2/3 complex-dependent endocytosis

This study shows lithium induces ciliary elongation via Arp2/3 complex-dependent endocytosis, providing additional ciliary membrane.

ERK pathway activation inhibits ciliogenesis and causes defects in motor behavior, ciliary gating, and cytoskeletal rearrangement

Initial ciliary assembly in Chlamydomonas requires Arp2/3 complex-dependent endocytosis

The paper shows Arp2/3 complex-dependent endocytosis is required for initial ciliary assembly in Chlamydomonas by reclaiming plasma membrane proteins.

Separable roles for RanGTP in nuclear and ciliary trafficking of a kinesin-2 subunit

This study shows RanGTP regulates ciliary trafficking of KAP3 independently of its nuclear roles, using Chlamydomonas and mammalian cells.

Chlamydomonas reinhardtii formin FOR1 and profilin PRF1 are optimized for acute rapid actin filament assembly

This study identifies FOR1 and PRF1 in Chlamydomonas reinhardtii as key regulators of rapid F-actin assembly for fertilization tubule formation.

The elusive actin cytoskeleton of a green alga expressing both conventional and divergent actins

The paper visualizes and characterizes filamentous actin structures in Chlamydomonas reinhardtii, revealing a perinuclear ring formed by an actin isoform insensitive to depolymerization.

Partially redundant actin genes in Chlamydomonas control flagellum-directed traffic and transition zone organization.

This study shows that actin redundancy in Chlamydomonas is essential for flagellar assembly, with NAP1 compensating for conventional actin in vesicle trafficking and transition zone organization.

Flagellar synchronization is a simple alternative to cell cycle synchronization for ciliary and flagellar studies

This paper introduces a method to synchronize flagellar length in Chlamydomonas reinhardtii without cell cycle synchronization, enabling precise study of flagellar regulation.