Pauline Mayonove

CF2H: a Cell-Free Two-Hybrid platform for rapid protein binder screening

CF2H is a cell-free two-hybrid platform that rapidly detects high-affinity protein-protein interactions without cloning or purification.

Split minireporters facilitate monitoring of gene expression and peptide production in linear cell-free transcription-translation systems

This paper introduces minireporters for monitoring gene expression and peptide production in cell-free systems, reducing DNA length and synthesis costs while maintaining reliable output.

Rapid prototyping of metabolites detection by bacterial biosensors in human fecal samples.

This paper develops a method to detect gut metabolites in human fecal samples using bacterial biosensors, showing their sensitivity and the need for individual optimization.

Rational programming of history-dependent logic in cellular populations.

This paper presents systematic and automated frameworks for implementing robust history-dependent genetic programs in cellular populations.

Hierarchical composition of reliable recombinase logic devices.

This paper presents a framework for building reliable recombinase logic devices using hierarchical composition of optimized switches, enabling up to 4-input Boolean logic control of cellular behavior.

Scalable composition frameworks for multicellular logic

This paper presents scalable frameworks for designing multicellular consortia that perform Boolean and history-dependent logic using recombinase-based systems, without requiring cell-cell communication or spatial separation.