R. Kiewisz

MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles

This study uses electron tomography to show that MT minus ends in mitotic spindles are heterogeneous, with MCRS1 modulating their open/closed states.

Three-dimensional structure of the kinetochore-fibers in human mitotic spindles

This paper uses electron tomography to reveal the 3D structure of kinetochore-fibers in human mitotic spindles, showing nine KMTs per fiber and structural variations along their length.

Self-organization of kinetochore-fibers in human mitotic spindles

This study investigates how kinetochore microtubules (KMTs) organize in human mitotic spindles using electron tomography, experiments, and modeling, finding that KMTs grow along defined trajectories and predominantly nucleate de novo at kinetochores.

Semi-automatic stitching of filamentous structures in image stacks from serial-section electron tomography

The paper presents a semi-automatic workflow for aligning and stitching filamentous structures in serial-section electron tomography data to improve 3D reconstruction of microtubules in spindles.

Male meiotic spindle features that efficiently segregate paired and lagging chromosomes

The paper identifies mechanisms in C. elegans male meiosis that enable efficient segregation of paired and lagging chromosomes during anaphase.

Central spindle microtubules are strongly coupled to chromosomes during both anaphase A and anaphase B

The paper measures central spindle microtubule movement during chromosome segregation in human and C. elegans spindles, finding they slide apart at the same speed as chromosomes and are strongly coupled to them.