Author

R. N. Gunawardane

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Cell Biology· 5

Signature Assays, Organisms & Methods

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Organism:human· 5 Research Focus:cell type annotation· 4 Approach:genetic perturbation / CRISPR· 2 Assay:scRNA-seq· 2 Approach:statistical model· 2 Research Focus:clustering· 1 Assay:CRISPR screen· 1 Approach:deep learning· 1

Frequent Co-Authors

A. Nelson· 4 shared J. Arakaki· 4 shared S. M. Rafelski· 4 shared C. Yan· 3 shared K. A. Gerbin· 3 shared M. C. Hendershott· 3 shared T. Grancharova· 3 shared A. B. Rosenberg· 2 shared

Activity by Year

2017-2021 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 41 mutual co-author links

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4 shared papersCell Biology

A. Nelson

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5 papers

Recent·Most cited

Cell BiologybioRxivApr 22, 2021

A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes

T. Grancharova, K. A. Gerbin, A. B. Rosenberg…·R. N. Gunawardane

The paper analyzes gene expression changes in hiPSC-derived cardiomyocytes using single-cell RNA-seq, identifying key genes that mark differentiation stages and sources of variation across protocols and cell lines.

DOI 10.1038/s41598-021-94732-1

humanscRNA-seq

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Cell BiologybioRxivPreprintDec 18, 2020

Automated hiPSC culture and sample preparation for 3D live cell microscopy

M. E. Coston, B. W. Gregor, J. Arakaki…·N. Gaudreault

The paper describes an automated system for hiPSC culture and sample preparation to enable standardized 3D live cell imaging and analysis.

DOI 10.1101/2020.12.18.423371

humanimaging

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Cell BiologybioRxivMay 26, 2020

Cell states beyond transcriptomics: integrating structural organization and gene expression in hiPSC-derived cardiomyocytes

K. A. Gerbin, T. Grancharova, R. Donovan-Maiye…·R. N. Gunawardane

The paper integrates gene expression and sarcomere organization in hiPSC-derived cardiomyocytes to predict subcellular states and reveal correlations between transcript abundance and structural patterns.

DOI 10.1016/j.cels.2021.05.001

humanscRNA-seq

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Cell BiologybioRxivJun 9, 2018

Scarless gene tagging of transcriptionally silent genes in hiPSCs to visualize cardiomyocyte sarcomeres in live cells

B. Roberts, J. Arakaki, K. A. Gerbin…·R. N. Gunawardane

The paper describes a CRISPR/Cas9 method to tag transcriptionally silent genes in hiPSCs with mEGFP, enabling visualization of sarcomeric proteins in live cardiomyocytes.

DOI 10.1016/j.stemcr.2019.03.001

humangenetic perturbation / CRISPR

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Cell BiologybioRxivMar 31, 2017

Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization

B. Roberts, A. Haupt, A. Tucker…·R. N. Gunawardane

The paper describes a CRISPR/Cas9 method to tag endogenous proteins in human iPSCs, generating 10 tagged lines with quality-controlled clones for cell biology research.

DOI 10.1091/mbc.e17-03-0209

humanCRISPR screen

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