Author

S. M. Rafelski

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Cell Biology· 7

Signature Assays, Organisms & Methods

Click to filter · or open tag

Research Focus:cell type annotation· 6 Organism:human· 6 Approach:statistical model· 3 Research Focus:cell signaling & trafficking· 2 Research Focus:clustering· 2 Approach:deep learning· 2 Approach:experimental assay· 2 Approach:genetic perturbation / CRISPR· 2

Frequent Co-Authors

M. C. Hendershott· 4 shared M. P. Viana· 4 shared R. N. Gunawardane· 4 shared A. Nelson· 3 shared I. A. Mueller· 3 shared J. Arakaki· 3 shared R. Yang· 3 shared A. Haupt· 2 shared

Activity by Year

2017-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 34 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

4 shared papersCell Biology

M. C. Hendershott

Mutual co-authors in cluster

Author profile →

7 papers

Recent·Most cited

Mitochondrial protein heterogeneity stems from the stochastic nature of co-translational protein targeting in cell senescence

A. H. Khan, R. J. Patel, M. P. Viana, S. M. Rafelski, A. I. Brown, B. M. Zid, T. Tsuboi

This study shows that mitochondrial fragmentation increases protein expression noise due to stochastic co-translational targeting, and that fission-fusion processes reduce this heterogeneity.

bioRxiv · Dec 3, 2023Cell Biology

Automated hiPSC culture and sample preparation for 3D live cell microscopy

M. E. Coston, B. W. Gregor, J. Arakaki, A. Borensztejn, T. P. Do, M. A. Fuqua, A. Haupt, M. C. Hendershott, W. Leung, I. A. Mueller, A. M. Nelson, S. M. Rafelski, M. J. Swain-Bowden, W. J. Tang, D. J. Thirstrup, , N. Gaudreault

The paper describes an automated system for hiPSC culture and sample preparation to enable standardized 3D live cell imaging and analysis.

bioRxiv · Dec 18, 2020 · PreprintCell Biology

The Allen Cell and Structure Segmenter: a new open source toolkit for segmenting 3D intracellular structures in fluorescence microscopy images

J. Chen, L. Ding, M. P. Viana, H. Lee, M. F. Sluezwski, B. Morris, M. C. Hendershott, R. Yang, I. A. Mueller, S. M. Rafelski

The paper introduces the Allen Cell and Structure Segmenter, an open-source toolkit for 3D segmentation of intracellular structures in fluorescence microscopy images, achieving high accuracy through classic and deep learning workflows.

bioRxiv · Dec 13, 2020 · PreprintCell Biology

Cell states beyond transcriptomics: integrating structural organization and gene expression in hiPSC-derived cardiomyocytes

K. A. Gerbin, T. Grancharova, R. Donovan-Maiye, M. C. Hendershott, J. Brown, S. Q. Dinh, J. L. Gehring, M. Hirano, G. R. Johnson, A. Nath, A. Nelson, C. M. Roco, A. B. Rosenberg, M. F. Sluzewski, M. P. Viana, , R. N. Gunawardane

The paper integrates gene expression and sarcomere organization in hiPSC-derived cardiomyocytes to predict subcellular states and reveal correlations between transcript abundance and structural patterns.

bioRxiv · May 26, 2020Cell Biology

Mitochondrial volume fraction and translation speed impact mRNA localization and production of nuclear-encoded mitochondrial proteins

T. Tsuboi, M. P. Viana, F. Xu, J. Yu, R. Chanchani, X. G. Arceo, E. Tutucci, J. Choi, Y. S. Chen, R. H. Singer, S. M. Rafelski, B. M. Zid

bioRxiv · Oct 10, 2019Cell Biology

Scarless gene tagging of transcriptionally silent genes in hiPSCs to visualize cardiomyocyte sarcomeres in live cells

B. Roberts, J. Arakaki, K. A. Gerbin, H. Malik, A. Nelson, M. C. Hendershott, C. Hookway, S. A. Ludmann, I. A. Mueller, R. Yang, S. M. Rafelski, R. N. Gunawardane

The paper describes a CRISPR/Cas9 method to tag transcriptionally silent genes in hiPSCs with mEGFP, enabling visualization of sarcomeric proteins in live cardiomyocytes.

bioRxiv · Jun 9, 2018Cell Biology

Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization

B. Roberts, A. Haupt, A. Tucker, T. Grancharova, J. Arakaki, M. A. Fuqua, A. Nelson, C. Hookway, S. A. Ludmann, I. M. Mueller, R. Yang, A. R. Horwitz, S. M. Rafelski, R. N. Gunawardane

The paper describes a CRISPR/Cas9 method to tag endogenous proteins in human iPSCs, generating 10 tagged lines with quality-controlled clones for cell biology research.

bioRxiv · Mar 31, 2017Cell Biology