Reprogramming the endogenous type III-A CRISPR-Cas system for genome editing, RNA interference and CRISPRi screening in Mycobacterium tuberculosis
K. Rahman, M. Jamal, X. Chen, W. Zhou, B. Yang, Y. Zou, W. Xu, Y. Lei, C. Wu, X. Cao, R. Tyagi, M. A. Naeem, D. Lin, Z. Habib, N. Peng, , G. Cao
This paper reprograms the endogenous CRISPR-Cas10 system in Mycobacterium tuberculosis for efficient gene editing, RNA interference, and genome-wide screening, enabling functional genomics studies and drug/vaccine development.
bioRxiv · Mar 9, 2020 · PreprintMicrobiology