Author

Ralfh Pulmones

Liverpool School of Tropical Medicine

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 3

Signature Assays, Organisms & Methods

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Organism:E. coli· 2 Approach:statistical model· 2 Research Focus:variant calling· 2 Assay:whole-genome sequencing· 2 Approach:experimental assay· 1 Assay:metagenomics· 1 Organism:mouse· 1

Frequent Co-Authors

Adam Paul RobertsiD· 3 shared Ellie AllmaniD· 2 shared Richard N. GoodmaniD· 2 shared Sabrina John MoyoiD· 2 shared Aakash KhanijauiD· 1 shared Alexander D. H. KingdoniD· 1 shared Amber Cutcliffe· 1 shared Amy McLemaniD· 1 shared

Activity by Year

2025-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 39 mutual co-author links

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3 shared papersMicrobiology

Adam Paul RobertsORCID

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

A citywide metagenomic analysis reveals surface-specific microbiome and resistome patterns in outdoor urban environments across Liverpool, UK.

Gavin Ackers-Johnson, Angus M. O’Ferrall, Andrew Holmes, Ellie Allman, Pauline Ambrose, Anya Breen, Amber Cutcliffe, Kara D’Arcy, Richard N. Goodman, Alexander D. H. Kingdon, Amy McLeman, Sabrina John Moyo, Ralfh Pulmones, Moon Deb Rajib, Priyanka Sharma, , Adam Paul Roberts

The paper uses shotgun metagenomic sequencing to analyze microbial communities and antimicrobial resistance genes across five urban surface types in Liverpool, finding distinct microbial and resistance profiles with higher ARG abundance on frequently contacted surfaces.

bioRxiv · Oct 8, 2026 · PreprintMicrobiology

In vivo de-amplification of a multi-resistance pseudo-compound transposon in Escherichia coli

Ralfh Pulmones, Sabrina John Moyo, Berhe Etsay Tesfay, Joshua G Gidabayda, Museveni Justine, Iren Høyland Löhr, Bjørn Blomberg, Simon C. Wagstaff, Nina Langeland, Adam Paul Roberts

This study tracks the in vivo evolution of an antibiotic-resistant E. coli strain in an infant gut, showing a reduction in IS26-mediated resistance gene arrays without fitness or susceptibility changes.

bioRxiv · Mar 6, 2026 · PreprintMicrobiology

From Colonisation to Invasion: Genomic and Phenotypic Comparison of Faecal and Bloodstream Isolates from the same patients

Aakash Khanijau, Ellie Allman, Ralfh Pulmones, Richard N. Goodman, Rachel J. McGalliard, Christopher M. Parry, Enitan D. Carrol, Adam Paul Roberts

This study compares genomic and phenotypic differences between faecal and bloodstream isolates from the same patients to understand pathogenesis of Gram-negative bloodstream infections.

bioRxiv · Jul 15, 2025 · PreprintMicrobiology