Accurate Genomic Variant Detection in Single Cells with Primary Template-Directed Amplification
Veronica Gonzalez-Pena, Sivaraman Natarajan, Yuntao Xia, David Klein, Robert Carter, Yakun Pang, Bridget Shaner, Kavya Annu, Daniel K. Putnam, Wenan Chen, Jon P. Connelly, Shondra M. Pruett‐Miller, Xiang 'Anthony' Chen, John B. Easton, Charles Gawad
This paper introduces PTA, a new WGA method that improves variant calling accuracy in single cells, enabling studies of genetic diversity and CRISPR off-target effects.
Proceedings of the National Academy of Sciences · Jun 7, 2021Peer-reviewedGenomics