Ruixin Wang

Improved, two-stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease

This paper describes a method to improve protein expression and purification in E. coli by inducing autolysis and DNA/RNA hydrolysis via phage lysozyme and endonuclease, achieving >90% protein release.

Improved, scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion.

This paper describes an improved method for recombinant protein expression in E. coli using phosphate depletion to trigger autoinduction, achieving high protein titers across various scales.