John S. Decker

This study reviews 168 publications on protein precipitation and liquid-liquid extraction, finding these methods are not always cheaper than chromatography and require optimization for cost-effectiveness.

This paper describes an improved bioprocess for producing Q-Griffithsin, a clinical-stage antiviral, using integrated autolysis, DNA hydrolysis, and precipitation to achieve high purity and yield without conventional chromatography.

This paper describes an improved method for recombinant protein expression in E. coli using phosphate depletion to trigger autoinduction, achieving high protein titers across various scales.