Tian Yang

This paper describes a method for rapidly producing and purifying Taq polymerase using engineered E. coli strains with autoinducible expression and simplified purification steps.

This paper introduces a multi-agent directed evolution approach that identifies hidden mutations by using multiple substrates, improving enzyme engineering efficiency.

This paper describes a method to improve protein expression and purification in E. coli by inducing autolysis and DNA/RNA hydrolysis via phage lysozyme and endonuclease, achieving >90% protein release.

This paper describes an improved method for recombinant protein expression in E. coli using phosphate depletion to trigger autoinduction, achieving high protein titers across various scales.