S. L. Keller

Several common methods of making vesicles (except an emulsion method) capture intended lipid ratios

This study compares lipid composition variations in vesicles made by five methods, finding significant differences in cholesterol and phospholipid ratios, particularly with emulsion transfer.

Sterol-lipids enable large-scale, liquid-liquid phase separation in bilayer membranes of only 2 components

The paper demonstrates that sterol-lipids can enable liquid-liquid phase separation in bilayer membranes with only two components, challenging previous requirements of three components.

Remodeling of yeast vacuole membrane lipidomes from the log (1-phase) to stationary stage (2-phases)

This paper investigates lipid composition changes in yeast vacuole membranes during growth to quiescence, finding increased phosphatidylcholine with higher melting temperatures.

Yeast cells actively tune their membranes to phase separate at temperatures that scale with growth temperatures

This study shows yeast cells regulate vacuole membrane phase separation by adjusting ergosterol levels to maintain a temperature offset from their growth temperature.

Proteins with prion-like domains can form viscoelastic condensates that enable membrane remodeling and endocytosis

The paper shows that prion-like domain-containing endocytic coat proteins form viscoelastic condensates that enable actin-independent membrane remodeling and endocytosis in yeast.

Ripples at edges of blooming lilies and torn plastic sheets

The paper derives a scaling relationship for ripples formed by excess length along edges in blooming lilies and torn plastic sheets, showing it applies broadly to similar systems.

Direct Imaging of Liquid Domains in Membranes by Cryo Electron Tomography

This paper develops two methods to directly image and quantify submicron liquid domains in membranes using cryo-electron tomography, enabling analysis under native conditions.