Multiplexed volumetric CLEM enabled by antibody derivatives provides new insights into the cytology of the mouse cerebellar cortex
Xiaomeng Han, Xiaotang Lu, Peter H. Li, Shuohong Wang, Richard Lee Schalek, Yaron Meirovitch, Zudi Lin, Jason Ken Adhinarta, Daniel Raimund Berger, Yuelong Wu, Tao Fang, Elif Sevde Meral, Shadnan Asraf, Hidde L. Ploegh, Hanspeter Pfister, , Jeff William Lichtman
The paper introduces a vCLEM method using fluorescent scFvs to combine molecular labeling with ssEM, revealing new cell types and subcellular features in mouse cerebellar cortex.
bioRxiv · May 20, 2023 · PreprintNeuroscience