James S. Trimmer

The paper develops methods to improve the solubility and stability of intrabodies by optimizing linker design, domain orientation, and charge properties, enabling effective intracellular targeting of cytoplasmic proteins.

The paper identifies a specific Kv2/Kv6.4 channel subtype in spinal motor neurons localized at C-bouton synapses, showing dependence on Kv2 subunits for expression and clustering.

The paper identifies Kv5.1 as a component of native Kv2.1 channels in mouse brain and shows it affects channel function and clustering.

The paper develops a screening strategy and database of antibodies validated for array tomography-based imaging of brain synapses, improving antibody selection for this technique.

NeuroMabSeq sequences hybridoma cells to generate recombinant monoclonal antibodies and scFvs for neuroscience research, making them publicly available.

The paper introduces a vCLEM method using fluorescent scFvs to combine molecular labeling with ssEM, revealing new cell types and subcellular features in mouse cerebellar cortex.

This paper identifies a KCNB1 pathogenic variant in mice that causes epilepsy and neurobehavioral abnormalities, mimicking human DEE features.

The paper develops cell body-targeted calcium indicators to improve signal fidelity in one-photon imaging of neural activity in live mice and zebrafish.

The paper identifies Kv2.1's role in clustering and functional coupling of L-type Ca2+ channels and RyR channels at ER-PM junctions, enabling localized Ca2+ release independent of action potentials.

The paper develops and validates a set of nanobodies for targeting proteins in brain neurons, providing a toolbox for visualization and modulation of subcellular sites.

The paper describes a method to generate and validate recombinant monoclonal antibodies for neuroscience research, enabling multiplex immunolabeling of brain tissue.

The paper introduces a framework for characterizing and screening antibodies against synaptic molecules for array tomography, providing objective metrics to evaluate antibody performance.