Hidde L. Ploegh

A pair of congenic mice for imaging of transplants by positron emission tomography using anti-transferrin receptor nanobodies

This paper develops a pair of congenic mice for PET/CT imaging of transplants using anti-TfR nanobodies, showing their ability to track transplanted cells without genetic modification.

Nanobody-targeted conditional antimicrobial therapeutics

This study designs nanobody-targeted conditional antimicrobial therapeutics to deliver therapeutic cargos to bacterial infection sites by enhancing protease activation through proximity-enabled reactivity.

Mapping Alzheimer Disease Molecular Pathologies in Large-Scale Connectomics Data: A Publicly Accessible Correlative Microscopy Resource

The paper presents a vCLEM protocol to map Aβ and pTau in AD mouse hippocampus connectomics data, revealing new molecular and structural alterations.

Multiplexed volumetric CLEM enabled by antibody derivatives provides new insights into the cytology of the mouse cerebellar cortex

The paper introduces a vCLEM method using fluorescent scFvs to combine molecular labeling with ssEM, revealing new cell types and subcellular features in mouse cerebellar cortex.

Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies

The paper introduces two short NanoTag epitopes and their nanobodies for in vivo protein visualization and manipulation in Drosophila, demonstrating their utility in multiple assays and CRISPR-based tagging.

Deletion of the AMPylase mFICD alters cytokine secretion and affects cognitive plasticity in vivo

This study shows that deleting mFICD in mice affects cytokine secretion and improves cognitive plasticity in older mice.