Author

T. M. D. Sheard

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 4 Cell Biology· 2

Signature Assays, Organisms & Methods

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Assay:imaging· 5 Approach:microscopy & imaging analysis· 4 Research Focus:cell type annotation· 3 Research Focus:cell signaling & trafficking· 2 Approach:experimental assay· 2 Organism:human· 2 Organism:rat· 2 Research Focus:spatial domain detection· 2

Frequent Co-Authors

I. Jayasinghe· 6 shared E. White· 3 shared M. E. Hurley· 3 shared D. Steele· 2 shared K. M. Suen· 2 shared M. E. Spencer· 2 shared R. S. Seehra· 2 shared T. Shakespeare· 2 shared

Activity by Year

2020-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 23 mutual co-author links

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6 shared papersBiophysics

I. Jayasinghe

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Author profile →

6 papers

Recent·Most cited

Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy

T. M. D. Sheard, T. Shakespeare, R. S. Seehra, M. E. Spencer, K. M. Suen, I. Jayasinghe

The paper evaluates 14 fluorescent dye esters for their ability to label human sub-cellular compartments using expansion microscopy, finding differential staining patterns and specificity influenced by dye properties and experimental conditions.

bioRxiv · Feb 21, 2023Cell Biology

Geometry-preserving Expansion Microscopy microplates enable high fidelity nanoscale distortion mapping

R. S. Seehra, B. H. K. Allouis, T. M. D. Sheard, M. E. Spencer, T. Shakespeare, A. J. Cadby, I. Jayasinghe

This paper introduces a microplate system for expansion microscopy that preserves gel geometry and enables distortion mapping in HeLa cells.

bioRxiv · Feb 20, 2023Biophysics

Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease

M. E. Hurley, E. White, T. M. D. Sheard, D. Steele, I. Jayasinghe

The paper correlates cardiac calcium sparks with RyR clustering patterns using super-resolution microscopy in healthy and failing rat ventricular myocytes, revealing a link between spark properties and RyR organization.

bioRxiv · Feb 12, 2023 · PreprintBiophysics

Expansion microscopy reveals subdomains in C. elegans germ granules

K. M. Suen, T. M. D. Sheard, C.-C. Lin, D. Milonaityte, I. Jayasinghe, J. E. Ladbury

This study uses expansion microscopy to reveal subdomains within C. elegans P granules and their relationship to other germ granules.

bioRxiv · May 29, 2022Cell Biology

3D visualisation of the cardiac ryanodine receptor clusters and the molecular-scale fraying of dyads

T. M. D. Sheard, M. E. Hurley, A. J. Smith, J. Colyer, E. White, I. Jayasinghe

This study uses 3D visualization to examine RyR cluster structure and JPH2 sub-domains in cardiac dyads, revealing fragmentation in disease states.

bioRxiv · Oct 21, 2021 · PreprintBiophysics

A super-resolution protocol to correlate structural underpinnings of fast second-messenger signalling in primary cell types

M. E. Hurley, T. M. D. Sheard, R. Norman, H. M. Kirton, S. S. Shah, E. Pervolaraki, Z. Yang, N. Gamper, E. White, D. Steele, I. Jayasinghe

The paper describes a correlative imaging protocol to visualize calcium signals and ryanodine receptors at nanoscale resolution in primary cells.

bioRxiv · Sep 29, 2020Biophysics