T. Shimomura

Conformational rearrangements in 2nd voltage sensor domain switch PIP2- and voltage-gating modes in two-pore channels

This study shows that conformational changes in the voltage-sensor domain of TPCs determine whether they are PIP2- or voltage-gated, and that these modes can be switched by small molecules.

A Simple Fluorescence Assay for Cystine Uptake via the xCT in Cells using Selenocystine and a Fluorescent Probe

This paper introduces a fluorescence assay to measure xCT-mediated cystine uptake using selenocystine and a fluorescent probe, and evaluates its activity in different cells and inhibitor efficiency.

Intrinsic Gating Behavior of Voltage-Gated Sodium Channels Predetermines Regulation by Auxiliary β-subunits

This study shows that the intrinsic movement order of voltage-sensing domains in Nav channels determines their susceptibility to regulation by β1 and β3 subunits, explaining variable regulatory effects across channel isoforms.

New Lipophilic Fluorescent Dyes for Exosome Labeling: Monitoring of Cellular Uptake of Exosomes

The paper introduces three new lipophilic fluorescent dyes for exosome labeling that overcome the limitations of PKH dyes by preventing aggregation and size shift during cellular uptake.

A native prokaryotic voltage-dependent calcium channel with a novel selectivity filter sequence

The paper identifies a native prokaryotic Cav channel, CavMr, and shows that a glycine residue in its selectivity filter determines Ca2+ selectivity, conserved in eukaryotic Cavs.