Taegeun Bae

High-fidelity CRISPR genome editing of single-nucleotide mutation with near-complementary guide RNA via enhanced target binding kinetics

This paper introduces a CRISPR-Cas9 editing strategy using near-complementary sgRNAs to specifically target single-nucleotide mutations without relying on PAM sequences, demonstrating its effectiveness on a cancer-related mutation.

DNA-dependent RNA cleavage by the Natronobacterium gregoryi Argonaute

The paper demonstrates that NgAgo from Natronobacterium gregoryi cleaves RNA instead of DNA, functioning as a DNA-guided endoribonuclease.