Index Switching Causes "Spreading-Of-Signal" Among Multiplexed Samples In Illumina HiSeq 4000 DNA Sequencing
Rahul Sinha, Geoff Stanley, Gunsagar Singh Gulati, Camille Ezran, Kyle J. Travaglini, Eric Wei, Charles K. F. Chan, Ahmad N. Nabhan, Tianying Su, Rachel M. Morganti, Stephanie D. Conley, Hassan Chaı̈b, Kristy Red‐Horse, Michael Thornton Longaker, Michael P. Snyder, , Irving L. Weissman
On patterned flow cells using exclusion amplification, up to 5-10% of reads are credited to the wrong sample in a pool, traced to free index primers surviving library cleanup.
bioRxiv · Apr 9, 2017 · PreprintMolecular Biology