Next-generation all-in-one CRISPR/Cas9 multiply-edited CD30CAR-T cells:Potency despite risk of translocations
Jan-Malte Kleid, Michael Damrat, Milita Darguzyte, Manuel Rhiel, Natascha E. Stumpf, Tabea Kleitke, Sandra Ammann, Tatjana I. Cornu, Fawad Khan, Tobias Wollmann, Sven Borchmann, Christof Scheid, Christianne de Lima Borges Moraes, Tobias Riët, Sabine Awerkiew, , Renata Stripecke
This study develops next-generation CD30CAR-T cells using CRISPR/Cas9 for multiplex editing, showing high potency but risks of translocations with combined knockouts.
bioRxiv · Feb 27, 2026 · PreprintCancer Biology