3' Nucleotide Asymmetry Directs miRNA Strand Selection
Jeffrey C. Medley, Sumire Kurosu Moriya, Huiwu Ouyang, Heather Crawshaw, Sarah Y. Zhang, Ganesh Prabhakar Panzade, Will J. Sydzyik, Joel T. Sydzyik, Mira Bhandari, Christopher M. Hammell, Anna Zinovyeva
The paper identifies 3' nucleotide asymmetry as a key factor in miRNA strand selection, showing its role in guiding the loading of the guide strand into miRISC in C. elegans and human cells.
bioRxiv · Oct 15, 2025 · PreprintMolecular Biology