A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi
C. N. Takacs, M. Scott, Y. Chang, Z. A. Kloos, I. Irnov, P. A. Rosa, J. Liu, C. Jacobs-Wagner
This paper develops a CRISPR interference platform for efficient gene downregulation in Borrelia burgdorferi, demonstrating >95% repression efficiency for multiple genes and revealing roles in motility and cell division.
bioRxiv · Oct 12, 2020Microbiology