C. Jacobs-Wagner

Glycogen phase separation drives macromolecular rearrangement and asymmetric division in Escherichia coli

The paper shows glycogen phase separation in E. coli causes asymmetric division and macromolecular rearrangement during growth phase transition.

Coupling of cell growth modulation to asymmetric division and cell cycle regulation in Caulobacter crescentus

This study shows that Caulobacter crescentus daughter cells have different growth rates due to a G1 phase slowdown, mediated by (p)ppGpp, linking growth modulation to asymmetric division and cell cycle regulation.

Genome concentration limits cell growth and modulates proteome composition in Escherichia coli

The paper shows genome concentration limits transcription and affects proteome composition in E. coli, with effects detectable in nutrient-rich conditions.

Cas9-mediated endogenous plasmid loss in Borrelia burgdorferi

This paper develops a Cas9-based method to remove specific endogenous plasmids from Borrelia burgdorferi and demonstrates its effectiveness in eliminating plasmids like lp25 and lp28-1.

Polyploidy, regular patterning of genome copies, and unusual control of DNA partitioning in the Lyme disease spirochete

This study shows that Borrelia burgdorferi is polyploid in culture and ticks, with regular genome copy spacing controlled by ParA/ParZ and ParB/SMC systems.

Connecting single-cell ATP dynamics to overflow metabolism, cell growth and the cell cycle in Escherichia coli

This study quantifies ATP dynamics in single E. coli cells and links them to growth rate, metabolism, and cell cycle, revealing that overflow metabolism drives ATP fluctuations and growth heterogeneity.

A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi

This paper develops a CRISPR interference platform for efficient gene downregulation in Borrelia burgdorferi, demonstrating >95% repression efficiency for multiple genes and revealing roles in motility and cell division.

Proximity labeling at non-centrosomal microtubule-organizing centers reveals VAB-10B and WDR-62 as distinct microtubule regulators

The paper identifies VAB-10B and WDR-62 as distinct regulators of non-centrosomal microtubules using proximity labeling in C. elegans.

Nucleoid size scaling and intracellular organization of translation across bacteria

This study shows that nucleoid size scales with cell size in bacteria, similar to eukaryotes, and links nucleocytoplasmic ratios to translation organization.

RNA polymerases display collaborative and antagonistic group behaviors over long distances through DNA supercoiling

The paper shows that RNA polymerases in E. coli exhibit collaborative and antagonistic behaviors due to DNA supercoiling during transcription.

Characterization of fluorescent proteins, promoters, and selectable markers for applications in the Lyme disease spirochete Borrelia burgdorferi

The paper develops and characterizes fluorescent proteins, antibiotic selection markers, and promoters for use in Borrelia burgdorferi to enhance genetic tools and study its biology.

Genome-wide phenotypic analysis of growth, cell morphogenesis and cell cycle events in Escherichia coli.

The paper performs a genome-wide screen of Escherichia coli single-gene knockouts to uncover genes involved in cell morphogenesis, growth, and cell cycle, revealing scaling relationships between nucleoid size and cell cycle events.

mTORC1 controls phase-separation and the biophysical properties of the cytoplasm by tuning crowding

The paper investigates how mTORC1 regulates macromolecular crowding and biophysical properties of the cytoplasm by using GEMs to measure diffusion and phase-separation changes.