BioengineeringbioRxivPreprint
Determine Protein Interaction Affinity without Purification Reveals a Two-enzyme Co-recognition of SUMOylation Substrate using Quantitative FRET (qFRET) Technology
Ling Jiang, Zhehao Xiong, Yan Liu…·Jiayu Liao
This paper develops a high-throughput qFRET method to determine protein interaction affinity without purification, revealing a two-enzyme co-recognition mechanism for SUMOylation substrates.
DOI 10.1101/2025.07.29.667540