Determine Protein Interaction Affinity without Purification Reveals a Two-enzyme Co-recognition of SUMOylation Substrate using Quantitative FRET (qFRET) Technology
Ling Jiang, Zhehao Xiong, Yan Liu, Runrui Dang, Jun Li, Jiayu Liao
This paper develops a high-throughput qFRET method to determine protein interaction affinity without purification, revealing a two-enzyme co-recognition mechanism for SUMOylation substrates.
bioRxiv · Jul 29, 2025 · PreprintBioengineering