Michael McGregor

This paper describes a method to efficiently generate genetically modified primary human myeloid cells using CRISPR-Cas9 RNP nucleofection, demonstrating its utility in studying HIV-1 infection and myeloid cell biology.

Affinity-purification mass spectrometry of 26 SARS-CoV-2 proteins in HEK293T cells yields 332 high-confidence host interactions and 67 druggable human proteins with known compounds.

The paper describes a CRISPR-Cas9 platform for editing primary CD4+ T cells to study HIV host factors, enabling high-throughput gene manipulation and downstream analysis.