Jorge García‐Marqués

The paper reveals that early multipotent radial glial progenitors generate all projection neurons through parallel sublineages and fate-restricted daughter cells, challenging existing models of cortical development.

TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages to study birth-order-dependent cell fates.

The paper introduces three simplified CRISPR-based methods for DNA insertion in Drosophila, using short homology arms and ssDNA/dsDNA donors to generate transgenic constructs efficiently.

The paper introduces CaSSA, a CRISPR/Cas9-based platform for creating unlimited genetic switches to enable cell-type-specific genetic manipulation.

CAMIO enables high-throughput deletion analysis of native DNA sequences in multicellular organisms by using CRISPR to generate diverse mutations in offspring.

CLADES uses CRISPR/Cas9 to create a programmable sequence of reporters for lineage analysis, tracking cell birth order and progression across generations.

This paper analyzes CRISPR-based cell lineage encoding and reconstruction, finding hierarchical clustering with shared edits is optimal and proposing improved encoding methods to increase trackable depth.