Minoru Koyama

A Multimodal Adaptive Optical Microscope For In Vivo Imaging from Molecules to Organisms

The paper introduces MOSAIC, a multimodal adaptive optical microscope that enables non-invasive imaging across biological scales in live specimens.

Multiplane HiLo microscopy with speckle illumination and non-local means denoising

This paper introduces a method to reduce speckle noise in multiplane HiLo microscopy using non-local means denoising, enabling fast, high-contrast volumetric imaging.

Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator

The paper identifies a mutation that enhances the sensitivity of a fluorescent voltage indicator, improving its ability to detect both spiking and subthreshold voltage signals in multiple organisms.

TEMPO: A system to sequentially label and genetically manipulate vertebrate cell lineages

TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages to study birth-order-dependent cell fates.

A general approach to engineer positive-going eFRET voltage indicators

The paper describes engineering eFRET voltage indicators with positive-going fluorescence responses by modifying microbial rhodopsins.

High-throughput cellular-resolution synaptic connectivity mapping in vivo with concurrent two-photon optogenetics and volumetric Ca2+ imaging

The paper develops a high-throughput method to map synaptic connectivity in zebrafish using two-photon optogenetics and volumetric Ca2+ imaging.

A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies

iAChSnFR, a fluorescent indicator built from a bacterial periplasmic binding protein, reports acetylcholine with large, fast signals in mouse, fish, fly and worm preparations.

Unlimited genetic switches for cell-type specific manipulation

The paper introduces CaSSA, a CRISPR/Cas9-based platform for creating unlimited genetic switches to enable cell-type-specific genetic manipulation.

Bright and photostable chemigenetic indicators for extended in vivo voltage imaging

The paper introduces a new genetically encoded voltage indicator, Voltron, which improves brightness and photostability for extended in vivo voltage imaging in mice, zebrafish, and fruit flies.

CLADES: a programmable sequence of reporters for lineage analysis

CLADES uses CRISPR/Cas9 to create a programmable sequence of reporters for lineage analysis, tracking cell birth order and progression across generations.

Chronology-based architecture of descending circuits that underlie the development of locomotor repertoire after birth.

The paper examines how new spinal pathways from hindbrain V2a neurons develop in larval zebrafish to support the emergence of diverse locomotor behaviors postnatally.

50 Hz volumetric functional imaging with continuously adjustable depth of focus

The paper introduces a CADoF Bessel beam module for volumetric functional imaging at 50 Hz volume rate in larval zebrafish.

Observing the Cell in Its Native State: Imaging Subcellular Dynamics in Multicellular Organisms

The paper develops a method to image subcellular dynamics in vivo using lattice light sheet microscopy and adaptive optics, revealing phenotypic diversity across organisms and developmental stages.

Video-rate volumetric functional imaging of the brain at synaptic resolution

This paper introduces a method to achieve video-rate volumetric imaging of brain activity at synaptic resolution using Bessel foci in two-photon microscopy.