Jonathan D. Zirin

This study uses in-situ glial cell-surface proteomics in Drosophila to identify DIP-β as a pro-longevity factor regulated by glial cells.

This paper develops PD-iPCR to detect low-abundance secreted proteins in Drosophila by using nanobodies and tagged proteins, enabling sensitive quantification of hormones like ImpL2.

The paper presents split-Gal4 driver lines targeting conserved signaling ligands in Drosophila to identify cells co-expressing pairs of ligands.

The paper introduces a modular CRISPR vector assembly system called Fragmid, enabling rapid creation and testing of CRISPR technologies through combinatorial design and pooled screening.

This paper develops a toolkit for dual control of gene expression in Drosophila using GAL4/UAS and LexA/QF systems, providing new vectors and fly lines for orthogonal gene regulation.

The paper develops new CRISPR-based constructs for Drosophila gene tagging, enabling efficient gene modification with a 70-80% success rate regardless of exon-intron structure.

The paper introduces two short NanoTag epitopes and their nanobodies for in vivo protein visualization and manipulation in Drosophila, demonstrating their utility in multiple assays and CRISPR-based tagging.

This study develops and optimizes CRISPR-Cas13 tools for RNA knockdown in Drosophila cells, improving efficiency and reducing toxicity.

The paper updates TRiP's RNAi and CRISPR fly stocks, presenting over 5,000 CRISPR-OE and -KO stocks for gene activation and knockout studies in Drosophila.

The paper introduces a homology-independent CRISPR-based method for efficient genomic knock-ins in Drosophila, demonstrating its use for fluorescent tagging and gene disruption.

The paper introduces three simplified CRISPR-based methods for DNA insertion in Drosophila, using short homology arms and ssDNA/dsDNA donors to generate transgenic constructs efficiently.

This paper introduces flySAM, a CRISPRa tool for Drosophila that improves effectiveness, scalability, and ease-of-use compared to existing methods.

The paper identifies genes involved in zinc detoxification in Drosophila cells through functional genomics and transcriptomics analyses, revealing key pathways and human disease-related gene orthologs.