Ching-Po Yang

Omega-seq: ultra-low-background RNA sequencing with faithful molecular counting and precise transcript-end capture

USER-excisable uracil TSOs, a uracil-intolerant polymerase and excess primer confine UMI incorporation to reverse transcription, minimizing background and UMI inflation; a separate Omega-shaped dT primer captures stranded transcript ends.

Imp1 acts as a dosage- and stage-dependent temporal rheostat orchestrating radial glial fate transitions and cortical morphogenesis

This study shows Imp1 regulates temporal fate transitions in radial glia during cortical development, affecting neuronal identity and spatial organization through dosage and stage-dependent mechanisms.

Driver lines for studying associative learning in Drosophila

The paper introduces a new collection of over 800 split-GAL4 and split-LexA drivers for studying associative learning in Drosophila, covering 300 cell types and enabling functional analysis of neural circuits.

Conservation and Divergence of Related Neuronal Lineages in the Drosophila Central Brain

The paper maps Drosophila central brain lineages to show how lineage identity and temporal patterning drive neuronal diversity and complexity.

Neuronal upregulation of Prospero protein is driven by alternative mRNA polyadenylation and Syncrip-mediated mRNA stabilisation

The paper shows that larval neurons upregulate Prospero protein through alternative polyadenylation and Syncrip-mediated mRNA stabilization.

Unlimited genetic switches for cell-type specific manipulation

The paper introduces CaSSA, a CRISPR/Cas9-based platform for creating unlimited genetic switches to enable cell-type-specific genetic manipulation.

CLADES: a programmable sequence of reporters for lineage analysis

CLADES uses CRISPR/Cas9 to create a programmable sequence of reporters for lineage analysis, tracking cell birth order and progression across generations.

Stem cell intrinsic, Seven-up-triggered temporal factor gradients diversify intermediate neural progenitors

The paper identifies temporal RNA-binding protein gradients in Drosophila neuroblasts that regulate intermediate neural progenitor fate.

Imp/Syp Temporal Gradients Govern Decommissioning Of Drosophila Neural Stem Cells

The paper shows that Imp and Syp RNA-binding protein gradients control the timing of Drosophila neuroblast decommissioning by regulating Prospero accumulation and mediator complex activity.