Tzumin Lee

The paper identifies and quantifies spurious UMIs generated during PCR in RNA sequencing, showing they inflate counts and distort fold-changes, and proposes experimental prevention.

USER-excisable uracil TSOs, a uracil-intolerant polymerase and excess primer confine UMI incorporation to reverse transcription, minimizing background and UMI inflation; a separate Omega-shaped dT primer captures stranded transcript ends.

This study shows Imp1 regulates temporal fate transitions in radial glia during cortical development, affecting neuronal identity and spatial organization through dosage and stage-dependent mechanisms.

The paper introduces a new collection of over 800 split-GAL4 and split-LexA drivers for studying associative learning in Drosophila, covering 300 cell types and enabling functional analysis of neural circuits.

The paper examines the developmental origins and metamorphic fates of neurons in the larval and adult mushroom body circuits of Drosophila to understand how a functional larval brain is formed from a reduced set of neurons.

TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages to study birth-order-dependent cell fates.

The paper develops a method to reconstruct dense neuronal lineages in Drosophila despite sparse sampling and suboptimal lineage encoding.

The paper maps Drosophila central brain lineages to show how lineage identity and temporal patterning drive neuronal diversity and complexity.

The paper shows that larval neurons upregulate Prospero protein through alternative polyadenylation and Syncrip-mediated mRNA stabilization.

The paper introduces CaSSA, a CRISPR/Cas9-based platform for creating unlimited genetic switches to enable cell-type-specific genetic manipulation.

CAMIO enables high-throughput deletion analysis of native DNA sequences in multicellular organisms by using CRISPR to generate diverse mutations in offspring.

This paper introduces Enhanced Golic+ (E-Golic+), an improved gene targeting method in Drosophila that achieves 100% recovery of correct gene targeting in male germ cells.

CLADES uses CRISPR/Cas9 to create a programmable sequence of reporters for lineage analysis, tracking cell birth order and progression across generations.

The paper introduces a robust method for reconstructing CRISPR and tumor lineages using depth metrics and improves upon existing metrics for lineage reconstruction.

This paper analyzes CRISPR-based cell lineage encoding and reconstruction, finding hierarchical clustering with shared edits is optimal and proposing improved encoding methods to increase trackable depth.

The paper identifies temporal RNA-binding protein gradients in Drosophila neuroblasts that regulate intermediate neural progenitor fate.

The paper shows that Imp and Syp RNA-binding protein gradients control the timing of Drosophila neuroblast decommissioning by regulating Prospero accumulation and mediator complex activity.

This paper develops an optimized CRISPR/Cas toolbox for efficient genome engineering in Drosophila, achieving high targeting efficiency and enabling precise genome modification.