Hui-Min Chen

USER-excisable uracil TSOs, a uracil-intolerant polymerase and excess primer confine UMI incorporation to reverse transcription, minimizing background and UMI inflation; a separate Omega-shaped dT primer captures stranded transcript ends.

The paper develops a method to reconstruct dense neuronal lineages in Drosophila despite sparse sampling and suboptimal lineage encoding.

The paper maps Drosophila central brain lineages to show how lineage identity and temporal patterning drive neuronal diversity and complexity.

CAMIO enables high-throughput deletion analysis of native DNA sequences in multicellular organisms by using CRISPR to generate diverse mutations in offspring.

This paper introduces Enhanced Golic+ (E-Golic+), an improved gene targeting method in Drosophila that achieves 100% recovery of correct gene targeting in male germ cells.

This paper develops an optimized CRISPR/Cas toolbox for efficient genome engineering in Drosophila, achieving high targeting efficiency and enabling precise genome modification.