Alexander S. Lee

This paper introduces Nuc-MS, a method to directly analyze chromatin reader-nucleosome interactions by disassembling complexes and identifying histone proteoforms involved in binding.

The paper investigates how nucleosome context influences chromatin reader binding preferences using biochemical and genomic approaches, revealing that multivalent engagement often has regulatory effects beyond simple additivity.

This study shows replication stress reduces CENP-A nucleosome stability in S phase by disrupting ATRX and DAXX function, leading to centromere instability.