Developmental BiologyDevelopmental CellPeer-reviewed
CRISPR-Cas13d induces efficient mRNA knock-down in animal embryos
Gopal Kushawah, Luis Hernández-Huertas, Joaquín Abugattas-Núñez del Prado…·Miguel A. Moreno-Mateos
This study demonstrates that CRISPR-Cas13d efficiently knocks down specific mRNAs in zebrafish, medaka, killifish, and mouse embryos, leading to significant transcript level reduction and embryonic phenotypes.