Orion GB Banks

This paper introduces SpLiT-ChEC, a method to simultaneously map DNA binding and nucleosome positioning by using SpyTag/SpyCatcher for targeted MNase digestion, and finds it provides precise temporal control for chromatin analysis.

The paper introduces a rapid, inexpensive protocol for mapping nucleosome positions in fungi and mammalian cells, validated across multiple species and compatible with high-throughput sequencing.

The paper shows Isw2 is a targeted nucleosome remodeler through interactions with sequence-specific factors, using conserved acidic residues in Itc1 to position nucleosomes precisely.

This paper develops and validates engineered chromatin remodeling proteins (E-ChRPs) that can reposition nucleosomes and arrays, and evict DNA-binding proteins, with versatile targeting options.