Michael J. Harms

This paper engineers affibodies with tunable affinities for VEGF and PDGF using computational design and yeast display to improve controlled protein release from hydrogels.

This study investigates whether zebrafish have calprotectin, finding no orthologs of mammalian S100A8 or S100A9 and no functional equivalents among zebrafish s100 proteins.

This study shows that recombinant S100A9 expressed in insect cells lacks TLR4 activation compared to E. coli-expressed S100A9, due to altered oligomerization.

This study identifies how S100A9 interacts with CD14 to activate TLR4, showing CD14-dependent TLR4 internalization and a dynamic binding site on CD14 that overlaps with LPS binding.

This paper introduces SpLiT-ChEC, a method to simultaneously map DNA binding and nucleosome positioning by using SpyTag/SpyCatcher for targeted MNase digestion, and finds it provides precise temporal control for chromatin analysis.

The paper shows that zebrafish fin regeneration size and shape depend on niche cells and skeletal geometry, not cell memories.

The paper shows that epistasis coefficients from linear models are unreliable and random, suggesting epistasis should be treated as quantifiable uncertainty in genotype-phenotype maps.

The paper estimates nonlinear scales in genotype-phenotype maps and finds significant high-order epistasis even after accounting for nonlinearity.