Laura E. McKnight

This paper introduces SpLiT-ChEC, a method to simultaneously map DNA binding and nucleosome positioning by using SpyTag/SpyCatcher for targeted MNase digestion, and finds it provides precise temporal control for chromatin analysis.

This study shows that Tup1 is essential for transcriptional repression in quiescent S. cerevisiae cells during glucose starvation.

The paper introduces a rapid, inexpensive protocol for mapping nucleosome positions in fungi and mammalian cells, validated across multiple species and compatible with high-throughput sequencing.

The paper shows Isw2 is a targeted nucleosome remodeler through interactions with sequence-specific factors, using conserved acidic residues in Itc1 to position nucleosomes precisely.

This paper develops and validates engineered chromatin remodeling proteins (E-ChRPs) that can reposition nucleosomes and arrays, and evict DNA-binding proteins, with versatile targeting options.