Joshua Hutchings

This study quantifies how CpG methylation affects chromatin mechanics, nucleosome wrapping, and condensate properties across multiple scales.

The paper describes a Kaggle challenge for particle picking in cryo-electron tomography, resulting in improved algorithms and highlighting the need for better annotation quality measures.

This paper introduces a crossed laser phase plate (xLPP) for cryoEM, demonstrating improved contrast and resolution in imaging biological samples.

This study uses in-cell cryo-electron tomography to compare how type I and type II kinase inhibitors affect LRRK2-I2020T filament formation and microtubule association in cells.

This study characterizes the molecular architecture of tunneling nanotubes (TNTs) using live-cell imaging and cryo-ET, revealing a complex structure with actin, microtubules, and organelles.

AreTomo3 and DenoisET form an automated GPU pipeline that reconstructs cryo-electron tomograms with tile-based contrast transfer function correction, screens their quality, and denoises them during acquisition.

The paper introduces PP7 virus-like particles as a standardized benchmark for cryo-electron tomography, enabling rapid validation and resolution assessment of tomographic pipelines.

The paper investigates the structure of chromatin condensates across scales and shows how nucleosome arrangement and histone interactions influence their physical properties.

This paper introduces nanogold probes for identifying proteins in live cells and cryo-ET tomograms, enhancing proteome accessibility for electron microscopy.

The paper develops methods to analyze chromatin condensates using cryo-electron tomography and deep learning, revealing nucleosome structures and interactions in both reconstituted and native systems.

This study uses cryo-ET to show that LRRK2 can form microtubule-bound filaments in its autoinhibited state, with structural differences compared to active-LRRK2 filaments.

This study uses structural biology to reveal the architecture of the nuclear basket in yeast and mammals, showing how Mlp/Tpr proteins form its structural components.