Michael K. Rosen

Amyloid stability and condensate material properties independently govern the hnRNPA2 condensate to fibril transition

This study shows that amyloid structure and condensate stability independently influence the hnRNPA2 condensate-to-fibril transition, revealing a structural framework for this process.

Near-atomistic simulations reveal the molecular principles that control chromatin structure and phase separation

This study introduces OpenCGChromatin, a model that simulates chromatin structure and phase separation, revealing how linker-DNA length and histone modifications affect chromatin organization.

Spindle pole proteins confine chromosomes to ensure their expulsion during female meiosis

The paper identifies ZYG-9/ch-TOG as a spindle pole protein that binds DNA and packages meiotic chromosomes into polar bodies, ensuring proper genome copy number in oocytes.

Electrostatic control of chromatin compaction safeguards against apoptotic DNA release

This study shows that electrostatic attraction compacts fragmented DNA during apoptosis, preventing its release, and demonstrates a synthetic approach to probe physical forces in genome organization.

Multi-scale structure of chromatin condensates rationalizes phase separation and material properties

The paper investigates the structure of chromatin condensates across scales and shows how nucleosome arrangement and histone interactions influence their physical properties.

ExoSloNano: Multi-Modal Nanogold Tags for identi-fication of Macromolecules in Live Cells & Cryo-Electron Tomograms

This paper introduces nanogold probes for identifying proteins in live cells and cryo-ET tomograms, enhancing proteome accessibility for electron microscopy.

Polo-Like Kinase 1 phosphorylation tunes the functional viscoelastic properties of the centrosome scaffold

This study shows that PLK-1 phosphorylation of SPD-5 modulates the viscoelastic properties of the PCM scaffold in C. elegans embryos, affecting its size, integrity, and function.

An electrostatic repulsion model of centromere organisation

This study identifies electrostatic repulsion as the mechanism driving centromere positioning at the chromatin surface, independent of condensin or microtubules.

Nucleosome Spacing Can Fine-Tune Higher Order Chromatin Assembly

The paper shows that nucleosome spacing affects chromatin phase separation stability and dynamics through inter- and intramolecular stacking trade-offs.

Quantitative Spatial Analysis of Chromatin Biomolecular Condensates using Cryo-Electron Tomography

The paper develops methods to analyze chromatin condensates using cryo-electron tomography and deep learning, revealing nucleosome structures and interactions in both reconstituted and native systems.