Jacob J. Elmer

The paper develops and validates an ultra-low-cost, open-source normothermic ex vivo organ perfusion platform for perfusing porcine kidneys and human organs.

The paper introduces a system to regulate plasmid DNA expression in eukaryotic cells by targeting methylated DNA using a fusion protein that binds to GATC motifs.

The paper shows that components from the c-myb transcriptional system can reactivate epigenetically silenced transgenes using CRISPR activation in mammalian cells.

This study enhances transgene expression by adding NF-Y and CTCF binding motifs to promoters, increasing luciferase expression 10-14 fold.