Dongfang Liu

This paper introduces PRISM, a method for high-plex spatial RNA imaging using color intensity barcodes, validated across multiple tissues and showing the role of CAFs in tumor microenvironments.

This study evaluates 18 cell segmentation methods on light microscopy and fluorescence images, finding attention-based models perform best and provides a pre-trained resource for segmentation.

GeneSegNet improves cell segmentation by integrating gene expression and imaging data, outperforming methods that neglect or underuse these inputs.