Madhuri Vangipuram

protoSpaceJAM automates and optimizes CRISPR/Cas9 gRNA and HDR donor design, improving knock-in efficiency through recoding mutations and customizable parameters.

This study validates that heterozygous NRXN1 deletions impair neurotransmitter release in human neurons across multiple labs, suggesting a human-specific synaptic dysfunction relevant for schizophrenia drug discovery.