Thomas Christian Südhof

The paper identifies nanoscale clustering of Latrophilin-3 as a mechanism driving excitatory synapse assembly, showing that clustering, not ligand binding, is critical for synapse formation.

The paper uses human iPSC-derived neurons to study WNV effects, identifying strain-specific neuroattenuation and pathogenic signatures not seen in conventional cell lines.

The paper reanalyzes original data and conducts new experiments to show that neurexins have distinct functions in different synapse types.

This study shows that deleting neuroligins from astrocytes does not affect synapse numbers or astrocyte structure in mice or human neuron co-cultures.

This study identifies teneurins as SPARCL1 receptors and shows that SPARCL1 enhances synapse formation by binding to teneurins via its follistatin-like domain and activating synapse formation via its Ca2+-binding domain.

This study shows that postsynaptic membrane fusion via Stx3 is essential for specific hippocampal computations like novelty processing and memory consolidation, but not for presynaptic representations.

The paper develops human tri-lineage brain microtissues composed of neurons, astrocytes, and microglia, showing they recapitulate cell-cell interactions and pathological processes.

This study shows that Lphn1 is present in both excitatory and inhibitory synapses but is not essential for excitatory transmission, instead contributing to inhibitory synapse formation and function.

This study shows that activity-dependent alternative splicing of Latrophilin-3 controls synapse formation through dual pathways involving GS/cAMP signaling and phase-separated protein scaffolds.

This study identifies a combinatorial splicing code in Neurexin-3 that regulates inhibitory synapse function through a trans-synaptic dystroglycan signaling loop.

The paper uses spatial and single-cell transcriptomics to identify memory-specific transcriptional signatures in neurons and astrocytes of the basolateral amygdala, revealing their role in long-term memory formation.

This study engineers novel adhesion molecules that drive synapse formation in cultured neurons by combining domains from bacterial proteins and synaptic cell-adhesion molecules.

The paper investigates the impact of the NLGN3 R451C mutation on human neurons, finding it reduces protein levels but enhances excitatory synapses, potentially contributing to ASD pathogenesis.

The paper shows that distinct neurexin-cerebellin complexes regulate AMPA- and NMDA-receptor responses differently across neural circuits without affecting synapse formation.

This study shows that calsyntenin-3 deletion in Purkinje cells reduces inhibitory synapses while increasing excitatory parallel-fiber synapses, suggesting a novel role in regulating excitatory/inhibitory balance.

This study uses a novel approach to block long-term potentiation and finds that it selectively enhances salience coding in hippocampal neurons without affecting spatial memory.

The paper reveals a cis/trans-protein interaction network that organizes reciprocal dendro-dendritic synapses by compartmentalizing neurexin-based trans-synaptic complexes.

This paper develops a method to generate neuronal cells from adult fibroblasts and uses it to study gene expression and epigenetic changes in 22q11.2 deletion syndrome.

The paper identifies synucleins as essential for vesicular exocytosis of endocannabinoids, linking their role in Parkinson's Disease to synaptic plasticity.

This study validates that heterozygous NRXN1 deletions impair neurotransmitter release in human neurons across multiple labs, suggesting a human-specific synaptic dysfunction relevant for schizophrenia drug discovery.

The paper identifies bi-allelic variants in TSPOAP1 as a cause of autosomal recessive dystonia, showing different clinical presentations based on variant type.

The paper reveals a nanoscale tripartite complex at glutamatergic synapses formed by Ca2+-channels, vesicles, and AMPA receptors, showing their functional coordination in neurotransmission.

The paper shows that co-expressing NGN2 with EMX1 generates homogeneous glutamatergic neurons from human pluripotent stem cells, with altered transcriptional output and chromatin binding compared to NGN2 alone.

This study shows that neurexin-1 in astrocytes and neurons forms distinct nanodomains at synapses, enabling compartment-specific signaling that differentially regulates synapse function.

The paper introduces a simple, stimulation-free Ca2+-imaging protocol with Matlab algorithms for analyzing network activity in cultured human and mouse neurons, showing differential changes in mutant neurons.

The paper creates a cell type atlas of the adult murine striatum using single-cell RNA-seq and identifies discrete and continuous cell identities within spiny projection neurons.

The paper identifies persistent transcriptional changes in diverse cells of the medial prefrontal cortex associated with remote memory, including neurons, astrocytes, and microglia.

The paper shows ApoE variants differentially affect neuronal signaling and synaptogenesis, mirroring their impact on Alzheimer’s disease risk.

The paper develops and validates a set of nanobodies for targeting proteins in brain neurons, providing a toolbox for visualization and modulation of subcellular sites.

The paper investigates cross-modal plasticity in mouse autism models, finding enhanced auditory responses in visual cortex linked to E/I circuit imbalance.